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IEAP - Institute of Experimental and Applied Physics CTU - Czech Technical University in Prague
CTU - Czech Technical University in Prague
Publication  > Articles in Impacted Journals  > 'Microradiography of biological samples with Timepix'
Microradiography of biological samples with Timepix

Dammer Jiří, Mgr. IEAP
Weyda František, doc. RNDr. CSc. Biology Centre of the Academy of Sciences of the Czech Republic, v. v. i.
Benes J. Charles University in Prague, First Faculty of Medicine
Sopko Vitek, Ing., Ph.D. IEAP
Jakůbek Jan, Ing. Ph.D. IEAP
Vondracek V. University Hospital Na Bulovce, Department of Radiological Physics, Prague, Czech Republic


Scientific journal
JINST 6 C11005 doi:10.1088/1748-0221/6/11/C11005


Microradiography is an imaging technique using X-rays in the study of internal structures of objects. This rapid and convenient imaging tool is based on differential X-ray attenuation by various tissues and structures within the biological sample. The non-absorbed radiation is detected with a suitable detector and creates a radiographic image. In order to detect the differential properties of X-rays passing through structures sample with different compositions, an adequate high-quality imaging detector is needed. We describe the recently developed radiographic apparatus, equipped with Timepix semiconductor pixel detector. The detector is used as an imager that counts individual photons of ionizing radiation, emitted by an X-ray tube FeinFocus with tungsten, copper or molybdenum anode. Thanks to the wide dynamic range, time over threshold mode — counter is used as Wilkinson type ADC allowing direct energy measurement in each pixel of Timepix detector and its high spatial resolution better than 1μm, the setup is particularly suitable for radiographic imaging of small biological samples. We are able to visualize some internal biological processes and also to resolve the details of insects (morphology) using different anodes. These anodes generate different energy spectra. These spectra depend on the anode material. The resulting radiographic images varies according to the selected anode. Tiny live insects are an ideal object for our studies.


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Cite article as:
J. Dammer, F. Weyda, J. Benes, V. Sopko, J. Jakůbek, V. Vondracek, "Microradiography of biological samples with Timepix", JINST 6 C11005 doi:10.1088/1748-0221/6/11/C11005 (2011)

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