New portable equipments for the transmission materials-sensitive X-ray radiography (including 3D reconstruction) and for the imaging X-ray fluorescence will be developed to investigate the fine art – particularly paintings. Simultaneously, today available portable analytical and optical instruments will be tested within the international co-operation. Both testing and development will lead to formulation of novel methodology for including these methods consecutively and meaningfully to the scope of pre-restoration, art-historic and detailed materials research of artworks, respectively. In a cultural heritage protection scope, all portable instruments will be conceived as to monitor the current state, to indicate the causes of damage and the extent of previous restoration/conservation interferences. Application of advanced non-invasive methods to the research will reduce its price, because operating costs of portable instruments are lower than those of laboratory instruments. It also will be less destructive in general, because any future eventual sampling will be reduced and planned efficiently with respect to the results of non-invasive studies. New detectors will allow more faithful and detailed view on internal structure of artworks and provide more efficient interrelation of technological and materials descriptions, respectively – both in situ, without any sampling and without necessity of their expensive transportation to specialized laboratories and studios. As a part of new infrastructure – portable laboratory – a consulting centre will also be established at the workplace of the applicant to help with the most effective scheduling of materials analyses in the field including wider application of non-invasive methods. From a technical point of view, there are no competitive solutions available today with comparable parameters. Czech Republic could therefore aspire to be one of leading countries providing future trends in applied research of cultural heritage.